Prenyltransferases from the flavedo of Citrus sinensis
Identifieur interne : 004773 ( Main/Exploration ); précédent : 004772; suivant : 004774Prenyltransferases from the flavedo of Citrus sinensis
Auteurs : Milton De La Fuente [Chili] ; Luz M. Pérez ; Uta Hashagen ; Liliana Chayet ; Cecilia Rojas ; Gloria Portilla [Chili] ; Osvaldo CoriSource :
- Phytochemistry [ 0031-9422 ] ; 1981.
Abstract
A prenyltransferase activity (EC 2.5.1.1) has been partially purified from the flavedo of Citrus sinensis with 30–40-fold purification and 35–60 % yield. The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2E,6E)- and (2Z,6E)-farnesyl pyrophosphate. The two C15-products are predominant. The E- and Z-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of Z-synthetase were obtained. Mg2 + is required for full activity. Mn2 + or Co2 + can replace Mg2 +. The ratio of E/Z-products formed is different for each cation. Mg2 + complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of E- and Z-products.
Url:
DOI: 10.1016/S0031-9422(00)98529-7
Affiliations:
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Pérez</name></author><author><name sortKey="Hashagen, Uta" sort="Hashagen, Uta" uniqKey="Hashagen U" first="Uta" last="Hashagen">Uta Hashagen</name></author><author><name sortKey="Chayet, Liliana" sort="Chayet, Liliana" uniqKey="Chayet L" first="Liliana" last="Chayet">Liliana Chayet</name></author><author><name sortKey="Rojas, Cecilia" sort="Rojas, Cecilia" uniqKey="Rojas C" first="Cecilia" last="Rojas">Cecilia Rojas</name></author><author><name sortKey="Portilla, Gloria" sort="Portilla, Gloria" uniqKey="Portilla G" first="Gloria" last="Portilla">Gloria Portilla</name></author><author><name sortKey="Cori, Osvaldo" sort="Cori, Osvaldo" uniqKey="Cori O" first="Osvaldo" last="Cori">Osvaldo Cori</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2</idno><date when="1981" year="1981">1981</date><idno type="doi">10.1016/S0031-9422(00)98529-7</idno><idno type="url">https://api.istex.fr/document/FC8C6D8B6AFB95315A87D1239907DDB8E4DA44D2/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000253</idno><idno type="wicri:Area/Istex/Curation">000253</idno><idno type="wicri:Area/Istex/Checkpoint">001835</idno><idno type="wicri:doubleKey">0031-9422:1981:De La Fuente M:prenyltransferases:from:the</idno><idno type="wicri:Area/Main/Merge">004C18</idno><idno type="wicri:Area/Main/Curation">004773</idno><idno type="wicri:Area/Main/Exploration">004773</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Prenyltransferases from the flavedo of Citrus sinensis</title><author><name sortKey="De La Fuente, Milton" sort="De La Fuente, Milton" uniqKey="De La Fuente M" first="Milton" last="De La Fuente">Milton De La Fuente</name><affiliation wicri:level="1"><country xml:lang="fr">Chili</country><wicri:regionArea>Dep. de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Casilla 233, Santiago 1</wicri:regionArea><wicri:noRegion>Santiago 1</wicri:noRegion></affiliation></author><author><name sortKey="Perez, Luz M" sort="Perez, Luz M" uniqKey="Perez L" first="Luz M." last="Pérez">Luz M. 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The enzyme catalyses the condensation of IPP with DMAPP or GPP. The products are neryl and geranyl pyrophosphate as well as (2E,6E)- and (2Z,6E)-farnesyl pyrophosphate. The two C15-products are predominant. The E- and Z-synthetase activities are partially dissociated during the purification procedure, as well as by heat or ageing. Preparations devoid of Z-synthetase were obtained. Mg2 + is required for full activity. Mn2 + or Co2 + can replace Mg2 +. The ratio of E/Z-products formed is different for each cation. Mg2 + complexes of allylic substrates or of products protect the enzyme against heat-inactivation and against inactivation by DTNB. The results are interpreted in terms of two or more prenyltransferases stereoselective for the synthesis of E- and Z-products.</div></front></TEI><affiliations><list><country><li>Chili</li></country></list><tree><noCountry><name sortKey="Chayet, Liliana" sort="Chayet, Liliana" uniqKey="Chayet L" first="Liliana" last="Chayet">Liliana Chayet</name><name sortKey="Cori, Osvaldo" sort="Cori, Osvaldo" uniqKey="Cori O" first="Osvaldo" last="Cori">Osvaldo Cori</name><name sortKey="Hashagen, Uta" sort="Hashagen, Uta" uniqKey="Hashagen U" first="Uta" last="Hashagen">Uta Hashagen</name><name sortKey="Perez, Luz M" sort="Perez, Luz M" uniqKey="Perez L" first="Luz M." last="Pérez">Luz M. Pérez</name><name sortKey="Rojas, Cecilia" sort="Rojas, Cecilia" uniqKey="Rojas C" first="Cecilia" last="Rojas">Cecilia Rojas</name></noCountry><country name="Chili"><noRegion><name sortKey="De La Fuente, Milton" sort="De La Fuente, Milton" uniqKey="De La Fuente M" first="Milton" last="De La Fuente">Milton De La Fuente</name></noRegion><name sortKey="Portilla, Gloria" sort="Portilla, Gloria" uniqKey="Portilla G" first="Gloria" last="Portilla">Gloria Portilla</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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